Creating a baseline of physiological walking parameters for Balb/c and C57Bl/6 mice - a gait analysis study
Author(s):
Fritz T. (Germany)
,
Fritz T. (Germany)
Affiliations:
Histing Tina
,
Histing Tina
Affiliations:
Pohlemann T.
,
Pohlemann T.
Affiliations:
Klein M.
,
Klein M.
Affiliations:
Menger M.
,
Menger M.
Affiliations:
Meeß Jessica
,
Meeß Jessica
Affiliations:
Braun B.
,
Braun B.
Affiliations:
Laschke M.
Laschke M.
Affiliations:
ESSKA Academy. Fritz T. 05/09/18; 209259; P02-421
Dr. Tobias Fritz
Dr. Tobias Fritz
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Abstract
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Objectives: Studies using gait analysis in a clinical context, are already well established in current research. In animal research, in vivo gait analysis has also evolved in the last few years and is growing more important. Most published studies use the Catwalk gait analysis system, to assess neurological and pharmacological differences in rodent models. Using the Catwalk XT 10 (Noldus, Netherlands) a multitude of gait parameters can be assessed in real time.

The goal of this study was to analyze physiological walking patterns and parameters of different mouse stems, using female Balb/c and C57BL/6 mice with the same bodyweight (20g).

This study showed differences between both mouse, which results in significant differences between both mouse stems. This study shows that gait analysis in mice may be a helpful tool in the assessment of gait analysis, but also differences of mouse stems must be considered carefully in planning and comparing studies.

Methods: Animals
We used female Balb/c and C57BL/6 mice with an average of 4 months and a body weight of 20g ± 2 g.

Gait analysis
Gait analysis in mice was performed using an automated computer assisted method the Catwalk XT 10 as described in previous studies. The Catwalk consists of an elevated 1.3 m long glass plate which is illuminated by a green fluorescent light. A camera records the reflecting light when a mouse crosses the walkway and the paws have contact with the glass plate. Each mouse was placed individually and free to walk in the CatWalk walkway, without conditioning or any stimulus. We acquired 5 complient runs. All other Runs were excluded. The parameters were assessed on day 0 and 1. All data was recorded and analyzed by CatWalk XT 10. We analyzed both front and hind paws.

Data analysis
After testing the data for normal distribution and equal variance, differences between the two species were analyzed by the unpaired Student's t-test. All values are expressed as means ± SEM. Statistical significance was accepted for a value of p<0,05.

Results: Run Duration (s), Step Cycle (s): There were no significant differences between the mouse stems Balb/c and C57BL/6.
Mean Intensity of the 15 Most Intense Pixels (%), Max Contact Area (cm2),Duty Cycle (%) parameters showed significant higher findings in C57BL/6 compared to Balb/c mice at front and hind paws.
Print Area (cm²): There was a significant higher Print Area of all paws in the C57Bl/6 compared to Balb/c mice.

Conclusions: The CatWalk is an established and useful tool to analyze walking parameters in rodent studies. When comparing walking parameters of the mouse stems C57BL/6 and Balb/c physiological differences of the walking patterns were found between both mouse stems, which have to be considered when planning a study. There is still not much knowledge about physiological walking patterns and parameters of different mice stems. We think these differences have to be assessed and may be helpful to create a baseline for future studies.

Keywords:
Gait analysis, Catwalk XT, Physiological walking patterns
Objectives: Studies using gait analysis in a clinical context, are already well established in current research. In animal research, in vivo gait analysis has also evolved in the last few years and is growing more important. Most published studies use the Catwalk gait analysis system, to assess neurological and pharmacological differences in rodent models. Using the Catwalk XT 10 (Noldus, Netherlands) a multitude of gait parameters can be assessed in real time.

The goal of this study was to analyze physiological walking patterns and parameters of different mouse stems, using female Balb/c and C57BL/6 mice with the same bodyweight (20g).

This study showed differences between both mouse, which results in significant differences between both mouse stems. This study shows that gait analysis in mice may be a helpful tool in the assessment of gait analysis, but also differences of mouse stems must be considered carefully in planning and comparing studies.

Methods: Animals
We used female Balb/c and C57BL/6 mice with an average of 4 months and a body weight of 20g ± 2 g.

Gait analysis
Gait analysis in mice was performed using an automated computer assisted method the Catwalk XT 10 as described in previous studies. The Catwalk consists of an elevated 1.3 m long glass plate which is illuminated by a green fluorescent light. A camera records the reflecting light when a mouse crosses the walkway and the paws have contact with the glass plate. Each mouse was placed individually and free to walk in the CatWalk walkway, without conditioning or any stimulus. We acquired 5 complient runs. All other Runs were excluded. The parameters were assessed on day 0 and 1. All data was recorded and analyzed by CatWalk XT 10. We analyzed both front and hind paws.

Data analysis
After testing the data for normal distribution and equal variance, differences between the two species were analyzed by the unpaired Student's t-test. All values are expressed as means ± SEM. Statistical significance was accepted for a value of p<0,05.

Results: Run Duration (s), Step Cycle (s): There were no significant differences between the mouse stems Balb/c and C57BL/6.
Mean Intensity of the 15 Most Intense Pixels (%), Max Contact Area (cm2),Duty Cycle (%) parameters showed significant higher findings in C57BL/6 compared to Balb/c mice at front and hind paws.
Print Area (cm²): There was a significant higher Print Area of all paws in the C57Bl/6 compared to Balb/c mice.

Conclusions: The CatWalk is an established and useful tool to analyze walking parameters in rodent studies. When comparing walking parameters of the mouse stems C57BL/6 and Balb/c physiological differences of the walking patterns were found between both mouse stems, which have to be considered when planning a study. There is still not much knowledge about physiological walking patterns and parameters of different mice stems. We think these differences have to be assessed and may be helpful to create a baseline for future studies.

Keywords:
Gait analysis, Catwalk XT, Physiological walking patterns
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