Save
Staphylococcus Antimicrobial properties of antibiotic-loaded
ESSKA Academy. Ollivier m. May 12, 2018; 218109; FP41-1723
Assoc. Prof. matthieu Ollivier
Assoc. Prof. matthieu Ollivier
Login now to access Regular content available to all registered users.

You can access free non-premium educational content on the ESSKA Academy Portal by registering for free as 'ESSKA Academy User' here
Abstract
Discussion Forum (0)
Rate & Comment (0)
Objectives: Infection remains one of the top causes for failure and revision in hip and knee arthroplasties. As an increasing number of arthroplasties are completed with uncemented constructs if local antibiotics could be effectively and reliably be delivered through tantalum vehicles, there would be an opportunity to drastically reduce, prevent, and treat periprosthetic infections. It was our hypothesis that local antibiotics could be effectively and reliably be delivered through tantalum vehicles.

Methods: To perform this experimental ex-vivo study, Porous tantalum (TM) cylinders (1 cm3) were specifically designed. Eleven study groups were defined according to the type of bacteria Staphilococcus Aureus Methy-S (SAMS) or Methy-R (SAMR), and Staphylococcus Epidermidi (SE) and according to the tested antiobiotic: Vancomycine (Vanco), Tobramycine (Tobra) or Gentamycine (Genta). A controls groups was created using a phosphate buffered saline (PBS) serum. In each group, 5 samples were tested. The cylinders were bathed in an antiobiotic solution (50mg/mL for Vanco and Tobra and 40mg/mL) or PBS during one hour. The cylinders were then placed on an Agar Plate renewed every 24h and inoculated with the different bacteria and placed 37.2 degrees humid atmosphere. Zones of inhibition (ZOI) were measured each day (in mm) (including photographs) until no antibiotic activity (ZOI<20mm) is left based upon previously validated protocols.

Results: For the 3 bacteria, the TM cylinder with the PBS showed no antimicrobial properties (ZOI= 0 mm). For the SAMS, mean ZOI for TM+ Vanco were 38.6 mm at day 1 and progressively decrased to 17 mm at day 9, for TM+Genta: 50 mm at day1 and decreased to 23 at day10, for TM+Tobra: 51mm at day1 and decreased to 24 at day 7. For the SAMR, mean ZOI for TM+ Vanco were 34 mm at day1 and progressively decreased to 14.5 mm at day 5, for TM+Genta : 45 mm at day1 and decreased to 11 at day5, for TM+Tobra: 41mm at day1and decreased to 22.5 at day 3. For the SE, mean ZOI for TM+Vanco were 43 mm at day1 and progressively decreased to 23 mm at day 9, for TM+Genta : 56 mm at day1 and decreased to 24.5 at day10, for TM+Tobra: 57 mm at day1and decreased to 25.5 at day 7.

Conclusions: 
The results of our study confirmed our hypothesis. Elution experiment should performed to find the optimal loading protocol and then evaluate in vivo antimicrobial properties of loaded Tantalum.

Keywords:
total knee arthroplasty, tantalum, antimicrobial properties , antibiotic.
Code of conduct/disclaimer available in General Terms & Conditions
Anonymous User Privacy Preferences

Strictly Necessary Cookies (Always Active)

MULTILEARNING platforms and tools hereinafter referred as “MLG SOFTWARE” are provided to you as pure educational platforms/services requiring cookies to operate. In the case of the MLG SOFTWARE, cookies are essential for the Platform to function properly for the provision of education. If these cookies are disabled, a large subset of the functionality provided by the Platform will either be unavailable or cease to work as expected. The MLG SOFTWARE do not capture non-essential activities such as menu items and listings you click on or pages viewed.


Performance Cookies

Performance cookies are used to analyse how visitors use a website in order to provide a better user experience.



Google Analytics is used for user behavior tracking/reporting. Google Analytics works in parallel and independently from MLG’s features. Google Analytics relies on cookies and these cookies can be used by Google to track users across different platforms/services.


Save Settings