Biofilm different adhesion to silver and tin coated surfaces
ESSKA Academy. Conteduca F. 11/09/19; 286390
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Biofilm different adhesion to silver and tin coated surfaces

EKA Free Papers

Topic: TKA

Conteduca F., Drogo P., Andreozzi V., Caperna L., Ferretti A.
La Sapienza University of Rome, Sant Andrea Hospital, Rome, Italy

Introduction: Bacterial adhesion and grown on the prhosthesis surface and subsequent biofilm formation present a great danger to the long-term using of the biomaterials. A great effort has been paid to create antibacterial surfaces either to resist bacterial attachment or directly eliminate bacteria surrounding the implants.. The aim of this paper is to to compare bacteria behavior on silver and titanium surface (TiN).
Methods: Sterilized treated disk was colonized by a Escherichia coli (E.C.) bacterial isolate coming from a patient with prosthetic joint infection The bacterial strain was identified to species level by MALDI-typing and standard microbiological procedures. From an overnight suspension in BHI broth, a 1/100 diluition of E.C. isolate was performed in 25 mL of BHI and incubated at 37ºC until McFarland density of 0.4 had been reached (approximately 1.3 × 10^8 colony forming units/mL; exponential growth phase).
Results: During these experimental procedures, the mean number of E.C. CFUs detected after sonication using TIN and silver prosthesis disks were 11267±815 and 1594±110CFU/ml, respectively, extremely higher compared to those counted in the pre-sonication step with 475±60 CFU/ml and 120±73 CFU/ml. On the basis of these results, seems that silver surface is more suitable to procude less E.C. adhesiveness respect to TIN surface.
Regarding Staphylococcus epidermidis (S.E.) experiments, TIN and silver disks showed a bacterial load after sonication step approximately of 830±106 CFU/ml and 1785±312 CFU/ml respectively. Conversely, the number of bacteria counted in the pre-sonication step were approximately near to 0 CFU/ml with 6±11 CFU/ml and 0 CFU/ml for TIN and silver, respectively).
Discussion: Our study was undertaken to retrieve bacteria on titanium disk using an in-vitro model based on a final sonication step used to dislodge bacteria from biofilm. To quantify the presence of bacteria adhese to titanium surface we used the method described by Bjerkan G. et al.based on the concept that in order to detach bacteria from a biofilm, the number of bacteria recovered after sonication must be increased compared to what can be observed after the final step of repeated washing.
it seems that silver surface is more suitable to produce less E.C. adhesiveness respect to TIN surface.
These experiments demonstrate that, the silver coated surface increase S.E. adhesiveness and TIN showed the lower CFU/ml value than those calculated up to date.
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